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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was uncovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital function in endorsing mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are described. Two supplemental splice variants have been reported but in-depth nucleotide sequence data isn't still obtainable.

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Blocking peptides are peptides that bind exclusively to the goal antibody and block antibody binding. These peptide commonly consists of the epitope identified via the antibody.

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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the very best effects are...

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This guide outlines even more achievable optimization of cellular and immunoassay parameters to guarantee the best possible outcomes are...

Antibodies certain to the blocking peptide not bind towards the epitope about the concentrate on protein. By comparing the staining in the blocked antibody as opposed to the antibody by itself, you can see which staining is unique.

Target Details Cyclin E belongs to your highly conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Various cyclins exhibit unique expression and degradation styles which add into the temporal coordination of each and every mitotic event. Cyclin E sorts a posh with and features for a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S transition. This protein accumulates within the G1-S phase boundary which is degraded as cells development through S section.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...

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